Crossmember should stay in the same place, shifter linkage should all stay in the same place. You might need a bigger radiator to keep the V8 cool depending on what size you have now. You'll need the V8 fan shroud, and the throttle linkage cable will be shorter for the V8. Then of course the exhaust. I little bit of wiring will need to be done since the I6 igintion wires are much longer where they come out of the loom to the alternator.
We kinda like the 250 around here being that you can't kill it. Is yours Auto or Manual trans? A few around here will kill to have an I6 3 speed column shift. If it were me, I'd keep the I6 and find a Non Integral head off of an older 250 or 230 along with the intake and exhaust manifold.